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These bioactive components have wide ranging biological activities and cones- quently many different targets and mechanism of action. The potential beneficial effect of these compounds especially their antioxidant properties and their role in the prevention of non-communicable chronic diseases such as coronary heart disease, stroke, cancer and diabetes has been discussed.
Introduction dant activity and protects DNA damage , phenolic com- pounds have antioxidant and other important physiologi- The term pulses is limited to legume crops harvested so- cal and biological properties , saponins have hypocho- lely for dry grains and excludes legumes used for oil ex- lesterolaemic effect and anti-cancer activity .
The major pulses to human health derived from the consumption of pulse used for human consumption include chickpea Cicer ar- grains and examine the bioactivity of pulse lectins, phytic ietinumpigeon pea Cajanus cajanlentil Lens culna- acid, isoflavones, phytosterols and saponins, and their role risgreen gram mung bean Vigna radiatablack gram in the prevention of various chronic diseases.
Non-Nutritive Bioactive Components in bean Vigna lunatusadzuki bean Vigna angularisRice Pulses bean Vigna umbellatamoth bean Vigna acontifoliadry broad bean Vicia faba and dry cowpea Vigna ungui- The nutritional factors in pulse grains are widely consid- culata.
Recent researches have associated the consum- ered critical for human nutrition, however, the overwhel- ption of pulses with a decreased risk for a variety of ch- ming evidence from epidemiological studies indicate that ronic degenerative diseases such as cancer, obesity, dia- diets rich in pulse grains are also associated with a lower Science paper and cardiovascular diseases .
In addition which act as natural antioxidants and and protects DNA to the macronutrients, pulses contain a wide variety of damage Table 1. Enzyme Inhibitors pounds and saponins .
These nonnutritive bio-active Protein inhibitors of hydrolases present in pulses are ac- compounds earlier considered as anti-nutrients because tive against proteases, amylases, lipases, glycosidases, of their activity to reduce protein digestibility  and mi- and phosphatases.
From the nutritional aspect, the inhi- neral bioavailability have recently been shown to have bitors of the serine proteases trypsin and chymotrypsin health protective effects .
Phytic acid exhibits antioxi- are the most important . An Overview Table 1.
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Major non-nutritive bioactive phytochemicals in pulses. Can reduce the digestibility and biological value of dietary proteins.
Antioxidant activity and protects DNA damage 5 Phenolic compounds Antioxidant activity, Has been inversely associated with the risk of colon cancer 6 Phytosterols Lowers serum cholesterol level A potent calcium-activated potassium channel opener.
These compounds are also the Bowman-Birk family [10,11].
Protease inhibitor con- abundant as sterol glucosides and esterified sterol gluco- tent is moderate in kidney bean and cowpea 8 and Total phytosterol con- spectivelyand low in lupin seeds 1. Chickpea and pea contained high Lectins or haemagglutinins are found in most plant foods levels of campesterol Beans most species, including Pha- phytosterol level for kidney bean, with much lower con- seolus vulgaris seems to be important source of lectins.
The Lectins from some of the pulses can inhibit the growth of consumption of pulse grains has been reported to lower serum cholesterol and increase the saturation levels of cho- experimental animals and reduce the digestibility and bio- lesterol in the bile.
A dietary study conducted by Duane logical value of dietary proteins .
These antinutritio- , on humans over a seven week period showed that se- nal effects are most likely caused by some lectins that can rum LDL cholesterol was significantly lower during the impair the integrity of the intestinal epithelium and thus consumption of a diet consisting of beans, lentil, and field alter the absorption and utilization of nutrients.
Studies have suggested that lectins cholesterol levels, and plant sterols are increasingly incor- affect the immune response against ovalbumin and may porated into foods for this purpose .
Lectin is one of the major proteins found 2. Phytic Acid in lentil Lens culinaris. It accounts for about 2. An Overview as DNA repair, chromatin remodeling, endocytosis, nu- a high Total Antioxidant Capacity TAC probably rela- clear messenger RNA export and potentially hormone si- ted to the high content of condensed tannins present in gnaling important for plant and seed development .
The seed coat in lentil is very rich in catechins, is often regarded as an antinutrient because of strong mi- procyanidins dimers and trimers.
Until recently, phenolic compounds were regarded tance in addition to their beneficial effects in human diets as non-nutritive com- pounds and it was reported that ex- by acting as anticarcinogen  or by promoting health cessive content of polyphenols, in particular tannins, may in other ways such as in decreasing the risk of heart dis- have adverse consequences because it inhibits the bio- ease or diabetes .
Pulses have high content of phy- availability of iron and blocks digestive enzymes in the tate, which is located in the protein bodies in the endo- gastrointestinal tract . Phenolic compounds can also sperm. Raw lentil contained 0. The InsP6 concentration tends to be phenolics offer many health benefits and are vital in hu- higher in raw dry bean, blackeye pea, and pigeon pea than man nutrition .
High correlations between phenolic in lentil, green and yellow split pea, and chickpea and compositions and antioxidant activities have been repor- ranged between Pulses with highest total phenolic content lentil, red kidney and black bean exert the highest antioxidant chickpea, respectively .
In vivo and in vitro studies capacity assessed by 2,2-diphenylpicryhydrazyl DP- have demonstrated that inositol hexaphosphate InsP6, PH free radical scavenging, ferric reducing antioxidant phytic acid exhibits significant anticancer preventive as power FRAPand the oxygen radical absorbance capa- well as therapeutic properties .
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